True to its name, the simple stain is a very simple staining procedure involving single solution of stain place the stained smear on the microscope stage smear side up and focus the smear using the 10x objective diagnostic microbiology laboratory generally do not perform simple staining method. A video module on gram staining. Because microbial cytoplasm is usually transparent, it is necessary to stain microorganisms before they can be viewed with the light microscope in some cases, staining is unnecessary, for example when microorganisms are very large or when motility is to be studied, and a drop of the microorganisms can be placed directly. In addition to fixation, staining is almost always applied to color certain features of a specimen before examining it under a light microscope this species has not been successfully cultured in the laboratory on an artificial medium therefore, diagnosis depends upon successful identification using microscopic techniques. A gram stain the previous lab introduced simple staining techniques that enable microbiologists to observe the morphological characteristics of bacteria although simple stains are useful draw a picture of a typical microscopic field and identify both escherichia coli and staphylococcus epidermidis record this in the. Lab report the gram stain and simple stain smear purpose preparation of smear and application of simple stain to observe size,shape, arrangement, color of the microorganisms using oil immersion objectiveand application of to preparation of smear, firstly in aseptic conditions a sterile microscopic slide was taken. Mount, negative, gram, and endospore staining influence of different physical conditions title: gram stain procedure however, a brief discussion should be held before this experiment regarding the function and application of gram's stain we report a false-negative rate of gram-stain microscopy for septic agar and broth.
These stains will readily give up a hydroxide ion or accept a hydrogen ion, which leaves the stain positively charged since the surface of most bacterial cells is negatively charged, these positively charged stains adhere readily to the cell surface for more detailed information, consult page 93 of your lab manual. Simple staining in order to stain the bacterial specimen for microscopy one must first prepare the smear on the slide this basically involves 3 steps---- transferring a liquid suspension of the bacterium on the slide, drying the smear, and then heating slightly to firmly attach the smear to the slide once this is done, the. Experiment 1 wang yuyan bacterial stain living bacteria stain killed bacteria stain positive staining negative staining common stain single-dye stain experiment 1 wang yuyan report requires 1 objective 2 principles 3 materials 4 procedure 5 results (draw what you observed by the microscope. Lab report: isolation of pure culture, gram-staining, and microscopic observation 1 pharmaceutical sciences subject: microbiology lab report: isolation of pure culture, gram-staining, and microscopic observation prepared by: annisa hayatunnufus id number: 012014052438 (msu).
View lab report - differential staining lab report revised from scibls 101 at nc state harrington 1 differential staining of bacteria are classified as microorganisms and are thus microscopic (can only be seen using a microscope) organisms which also include fungi, protozoa, and viruses ninety- image of page 2. Who should do this evaluation the evaluator needs to have a good understanding of acid-fast bacilli (afb) smear microscopy and good laboratory practice (glp) methods assessed include specimen collection, smear preparation, acid-fast stain procedure, reading, and reporting the evaluator must understand. Cellular and molecular techniques – lab #1 bio 349 lab report #1 microscopy and staining abstract the primary focus of this lab was on microscopy and simple stains microscopy that was used were magnification, slide preparation, and staining methylene blue, a simple staining component, was used to stain the slide.
Discussion: after preforming gram-staining technique on the provided organisms, it was found that this experiment matched the results of previous gram staining results when observing e coli under the microscope it was apparent that the deep pink tint it obtained meant it was a gram negative cell, therefore having a thin. Human cheek cells experiment from microscopes for schools observing cells from a human cheek and bacteria under a compound microscope.
Observe the results of the staining procedure under oil immersion (100x) using a bright field microscope if you are struggling to remember the staining an urethral smear report might read: 'moderate numbers gram negative intracellular diplococci and many pus cells' quality control: always check new batches of stain. The report provides an interesting introductory guide to gram stain protocol for reporting critical calls (if applicable) lab 2: gram stain information gathered by: elizabeth rigg bunsen burner, alcohol-cleaned microscope slide, water example reports karen carroll, md, associate professor of disinfect. Laboratory testing – algorithms for use of nucleic acid amplification tests are often based on smear positivity • public health interventions – smear status and grade 1 preparing and fixing smears 2 staining smears 3 examining smears 4 recording and reporting results steps in performing afb microscopy 15.
Understanding the capabilities and limitations of the light microscope is important if one is to get the best results from microscopy the ability to identify structures and specific cell types has been greatly aided by different staining techniques and more recently by immunohistochemistry the investigation of cell structure has. Microscopy refers to the practice that involves the use of a microscope for the purposes of observing small scale structures that cannot be viewed using the naked eye and often cell staining is necessary as structures are difficult to discern due to insufficient contrast cell staining is a technique used for the main purpose of.
Lab #2: differential staining of bacteria lab powerpoint show lab #2: differential the lab exercise the lab report template is what the students use to develop the lab report for this project how to use a compound microscope laboratory exercise main page from the virtual microbiology classroom how to use a. Lisa ta february 29, 2012 biology 240 general microbiology mw 3:45-5:00 gram stain report introduction the purpose of this experiment is to determine the shape and gram stain of the bacteria under a microscope the reason for staining bacteria is due to the fact that most bacteria are transparent and cannot be. In a modern environmental or molecular microbiology lab, most identification is done using genetic sequences and other molecular techniques, which are far more specific and informative than differential staining gram staining has been suggested to be as effective a diagnostic tool as pcr in one primary research report. Microscope glass slide a collecting wire loop a heater (burner) sample (e coli culture) distilled water gram stain (crystal violet, iodine stain and carbol fuschin) using a scraper/laboratory scoop, dab vaseline on the corners of a cover slip using a wire loop, carefully place a loopful of the broth on to the center of the.